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ELK2997-48T, Rat COL1a1(Collagen Type I Alpha 1) ELISA Kit, 48T
2.142,00 RON
Rat COL1a1(Collagen Type I Alpha 1) ELISA Kit
SKU
ELK2997-48T
Alternative Names: COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
Species: Rat
Assay Type: Sandwich
Sensitivity: 13.7 pg/mL
Standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL1a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL1a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL1a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL1a1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain Species: Rat Assay Type: Sandwich Sensitivity: 13.7 pg/mL Standard: 2000 pg/mL Detection range: 31.25-2000 pg/mL Sample type: serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL1a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL1a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL1a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL1a1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
