ELK2989-48T, Human TMSb10(Thymosin Beta 10) ELISA Kit, 48T

Alternative Names: MIG12; TB10; PTMB10

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 13.52 ng/mL

Standard: 2000 ng/mL

Detection range: 31.25-2000 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 2h

Research Area: Endocrinology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TMSb10 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMSb10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMSb10 in the samples is then determined by comparing the OD of the samples to the standard curve.