ELK2988-48T, Human COL17(Collagen Type XVII) ELISA Kit, 48T

Alternative Names: COL17A1; BP180; BPAG2; LAD-1; LABD97; Collagen Alpha-1(XVII)chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; Linear IgA disease antigen 1

Species: Human

Assay Type: Sandwich

Sensitivity: 1.13 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Developmental science;Nutrition metabolism;Dermatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL17 in the samples is then determined by comparing the OD of the samples to the standard curve.