ELK2881-48T, Rat CX43(Connexin 43) ELISA Kit, 48T

Alternative Names: ODDD; GJAL; GJA1; GJ-A1; Gap junction 43 kDa heart protein; Gap Junction Protein Alpha 1; Oculodentodigital Dysplasia; Syndactyly Type III

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Cardiovascular biology;Neuro science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CX43. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CX43. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CX43, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CX43 in the samples is then determined by comparing the OD of the samples to the standard curve.