ELK2822-48T, Rabbit SOD3(Superoxide Dismutase 3, Extracellular) ELISA Kit, 48T

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ELK2822-96T, Rabbit SOD3(Superoxide Dismutase 3, Extracellular) ELISA Kit, 96T

3.736,60 RON

Rabbit SOD3(Superoxide Dismutase 3, Extracellular) ELISA Kit

SKU
ELK2822-96T

Alternative Names: SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 0.32 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit SOD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit SOD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit SOD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit SOD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: SOD-3; EC-SOD; Extracellular Superoxide Dismutase Cu-Zn

Species: Rabbit

Assay Type: Sandwich

Sensitivity: 0.32 ng/mL

Standard: 50 ng/mL

Detection range: 0.79-50 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Enzyme & Kinase;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit SOD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit SOD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit SOD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit SOD3 in the samples is then determined by comparing the OD of the samples to the standard curve.