ELK2762-96T, Human PPARd(Peroxisome Proliferator Activated Receptor Delta) ELISA Kit, 96T

Alternative Names: FAAR; NR1C2; NUC1; NUCI; PPARB; PPARδ/B; Nuclear Receptor Subfamily 1,Group C,Member 2; Nuclear hormone receptor 1; Peroxisome proliferator-activated receptor beta

Species: Human

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPARd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPARd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPARd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPARd in the samples is then determined by comparing the OD of the samples to the standard curve.