ELK2737-48T, Human TLR8(Toll Like Receptor 8) ELISA Kit, 48T

ELK2737-48T, Human TLR8(Toll Like Receptor 8) ELISA Kit, 48T

ELK2738-48T, Human TLR10(Toll Like Receptor 10) ELISA Kit, 48T

ELK2738-48T, Human TLR10(Toll Like Receptor 10) ELISA Kit, 48T

ELK2737-96T, Human TLR8(Toll Like Receptor 8) ELISA Kit, 96T

2.963,10 RON

Human TLR8(Toll Like Receptor 8) ELISA Kit

SKU
ELK2737-96T

Alternative Names: CD288

Species: Human

Assay Type: Sandwich

Sensitivity: 0.108 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Pulmonology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TLR8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TLR8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TLR8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TLR8 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CD288

Species: Human

Assay Type: Sandwich

Sensitivity: 0.108 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Pulmonology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TLR8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TLR8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TLR8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TLR8 in the samples is then determined by comparing the OD of the samples to the standard curve.