ELK2660-96T, Human MASP1(Mannose Associated Serine Protease 1) ELISA Kit, 96T

Alternative Names: CRARF; PRSS5; Mannan-Binding Lectin Serine Peptidase 1; Ra-reactive factor serine protease p100; Complement-activating component of Ra-reactive factor; Serine protease 5

Species: Human

Assay Type: Sandwich

Sensitivity: 42.57 pg/mL

Standard: 6000 pg/mL

Detection range: 93.75-6000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Apoptosis;Infection immunity;Hematology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.