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ELK2632-96T, Rat CCK(Cholecystokinin) ELISA Kit, 96T

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ELK2632-48T, Rat CCK(Cholecystokinin) ELISA Kit, 48T

2.142,00 RON

Rat CCK(Cholecystokinin) ELISA Kit

SKU
ELK2632-48T

Alternative Names: CCK-PZ

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 4.17 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Tumor immunity;Endocrinology;Neuro science;Hepatology;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat CCK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CCK in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: CCK-PZ

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 4.17 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 2h

Research Area: Tumor immunity;Endocrinology;Neuro science;Hepatology;Hormone metabolism;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat CCK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CCK in the samples is then determined by comparing the OD of the samples to the standard curve.