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ELK2623-48T, Rat APOB100(Apolipoprotein B100) ELISA Kit, 48T
2.142,00 RON
Rat APOB100(Apolipoprotein B100) ELISA Kit
SKU
ELK2623-48T
Alternative Names: Apo-B100
Species: Rat
Assay Type: Sandwich
Sensitivity: 4.9 ng/mL
Standard: 800 ng/mL
Detection range: 12.5-800 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOB100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOB100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOB100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Apo-B100 Species: Rat Assay Type: Sandwich Sensitivity: 4.9 ng/mL Standard: 800 ng/mL Detection range: 12.5-800 ng/mL Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOB100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOB100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOB100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve. |
