ELK2614-48T, Mouse S100A11(S100 Calcium Binding Protein A11) ELISA Kit, 48T

Alternative Names: S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11, N-terminally processed

Species: Mouse

Assay Type: Sandwich

Sensitivity: 2.97 pg/mL

Standard: 400 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length: 3.5h

Research Area: Tumor immunity;Infection immunity;Developmental science;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100A11 in the samples is then determined by comparing the OD of the samples to the standard curve.