ELK2564-96T, Mouse I-PTH(IntactParathormone) ELISA Kit, 96T

Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone

Species: Mouse

Assay Type: Sandwich

Sensitivity: 3.94 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.