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ELK2533-96T, Mouse MMP8(Matrix Metalloproteinase 8) ELISA Kit, 96T
2.963,10 RON
Mouse MMP8(Matrix Metalloproteinase 8) ELISA Kit
SKU
ELK2533-96T
Alternative Names: CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.121 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MMP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MMP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MMP8 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase Species: Mouse Assay Type: Sandwich Sensitivity: 0.121 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Serum, plasma and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase;Infection immunity;Cardiovascular biology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MMP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MMP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MMP8 in the samples is then determined by comparing the OD of the samples to the standard curve. |
