ELK2473-48T, Rat ANG2(Angiopoietin 2) ELISA Kit, 48T

ELK2473-48T, Rat ANG2(Angiopoietin 2) ELISA Kit, 48T

ELK2474-48T, Mouse ANG2(Angiopoietin 2) ELISA Kit, 48T

ELK2474-48T, Mouse ANG2(Angiopoietin 2) ELISA Kit, 48T

ELK2473-96T, Rat ANG2(Angiopoietin 2) ELISA Kit, 96T

2.963,10 RON

Rat ANG2(Angiopoietin 2) ELISA Kit

SKU
ELK2473-96T

Alternative Names: ANG2; AGPT2; ANGPT2

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.2 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ANG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ANG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ANG2 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: ANG2; AGPT2; ANGPT2

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.2 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ANG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ANG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ANG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ANG2 in the samples is then determined by comparing the OD of the samples to the standard curve.