ELK2467-96T, Rat MIF(Macrophage Migration Inhibitory Factor) ELISA Kit, 96T

Alternative Names: GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

Species: Rat

Assay Type: Sandwich

Sensitivity: 12.5 pg/mL

Standard: 2000 pg/mL

Detection range: 31.25-2000 pg/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Cytokine;Infection immunity;Rheumatology;Autoimmunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIF in the samples is then determined by comparing the OD of the samples to the standard curve.