Limba
Romanian
Romanian
English|
ELK2436-96T, Rat ET-1(Endothelin1) ELISA Kit, 96T
2.963,10 RON
Rat ET-1(Endothelin1) ELISA Kit
SKU
ELK2436-96T
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Species: Rat
Assay Type: Sandwich
Sensitivity: 2.71 pg/mL
Standard: 500 pg/mL
Detection range: 7.82-500 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1 Species: Rat Assay Type: Sandwich Sensitivity: 2.71 pg/mL Standard: 500 pg/mL Detection range: 7.82-500 pg/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Endocrinology;Cardiovascular biology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
