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ELK2411-48T, Human CRH(Corticotropin Releasing Hormone) ELISA Kit, 48T
2.142,00 RON
Human CRH(Corticotropin Releasing Hormone) ELISA Kit
SKU
ELK2411-48T
Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 4.91 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CRF; Corticotropin Releasing Factor; Corticoliberin Species: Human Assay Type: Competitive Inhibition Sensitivity: 4.91 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 2h Research Area: Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve. |
