ELK2401-48T, Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 48T

ELK2401-48T, Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 48T

ELK2402-48T, Dog TM(Thrombomodulin) ELISA Kit, 48T

ELK2402-48T, Dog TM(Thrombomodulin) ELISA Kit, 48T

ELK2401-96T, Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit, 96T

2.963,10 RON

Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit

SKU
ELK2401-96T

Alternative Names: NT-Pro-ANP; N-ANP

Species: Mouse

Assay Type: Sandwich

Sensitivity: 29 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Endocrinology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: NT-Pro-ANP; N-ANP

Species: Mouse

Assay Type: Sandwich

Sensitivity: 29 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Endocrinology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.