ELK2338-48T, Human IgG4(Immunoglobulin G4) ELISA Kit, 48T

Alternative Names: IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.073 µg/mL

Standard: 10 µg/mL

Detection range: 0.16-10 µg/mL

Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Immune molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG4 in the samples is then determined by comparing the OD of the samples to the standard curve.