ELK2252-48T, Human AP13(Apelin 13) ELISA Kit, 48T

ELK2252-48T, Human AP13(Apelin 13) ELISA Kit, 48T

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ELK2253-48T, Human CDK1(Cyclin Dependent Kinase 1) ELISA Kit, 48T

ELK2252-96T, Human AP13(Apelin 13) ELISA Kit, 96T

2.963,10 RON

Human AP13(Apelin 13) ELISA Kit

SKU
ELK2252-96T

Alternative Names: APLN13

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 8.24 pg/mL

Standard: 1600 pg/mL

Detection range: 25-1600 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: APLN13

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 8.24 pg/mL

Standard: 1600 pg/mL

Detection range: 25-1600 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 2h

Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP13 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP13. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.