ELK2250-48T, Human APOF(Apolipoprotein F) ELISA Kit, 48T

ELK2250-48T, Human APOF(Apolipoprotein F) ELISA Kit, 48T

ELK2251-48T, Human APOB48(Apolipoprotein B48) ELISA Kit, 48T

ELK2251-48T, Human APOB48(Apolipoprotein B48) ELISA Kit, 48T

ELK2250-96T, Human APOF(Apolipoprotein F) ELISA Kit, 96T

2.963,10 RON

Human APOF(Apolipoprotein F) ELISA Kit

SKU
ELK2250-96T

Alternative Names: Apo-F; LTIP; Lipid transfer inhibitor protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.41 ng/mL

Standard: 60 ng/mL

Detection range: 0.94-60 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Nutrition metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOF in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Apo-F; LTIP; Lipid transfer inhibitor protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.41 ng/mL

Standard: 60 ng/mL

Detection range: 0.94-60 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;Metabolic pathway;Endocrinology;Cardiovascular biology;Nutrition metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOF in the samples is then determined by comparing the OD of the samples to the standard curve.