Human AP36(Apelin 36) ELISA Kit
Alternative Names: APLN36
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.52 pg/mL
Standard: 600 pg/mL
Detection range: 9.38-600 pg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Endocrinology;Gastroenterology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP36 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP36. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP36 in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 1.800,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: APLN36 Species: Human Assay Type: Competitive Inhibition Sensitivity: 2.52 pg/mL Standard: 600 pg/mL Detection range: 9.38-600 pg/mL Sample type: Serum, plasma and other biological fluids Assay length: 2h Research Area: Metabolic pathway;Endocrinology;Gastroenterology; Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP36 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP36. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP36 in the samples is then determined by comparing the OD of the samples to the standard curve. |