ELK2238-96T, Human F7(Coagulation Factor VII) ELISA Kit, 96T

Alternative Names: FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa

Species: Human

Assay Type: Sandwich

Sensitivity: 3.5 pg/mL

Standard: 600 pg/mL

Detection range: 9.38-600 pg/mL

Sample type: Plasma

Assay length: 3.5h

Research Area: Metabolic pathway;Infection immunity;Hematology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F7 in the samples is then determined by comparing the OD of the samples to the standard curve.