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ELK2200-96T, Human PK(Pyruvate Kinase, Liver And RBC) ELISA Kit, 96T
2.963,10 RON
Human PK(Pyruvate Kinase, Liver And RBC) ELISA Kit
SKU
ELK2200-96T
Alternative Names: KLKB1; KLK3; Fletcher Factor; Kallikrein B,Plasma; Kininogenin; Plasma prekallikrein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.62 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Enzyme & Kinase;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PK in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: KLKB1; KLK3; Fletcher Factor; Kallikrein B,Plasma; Kininogenin; Plasma prekallikrein Species: Human Assay Type: Sandwich Sensitivity: 0.62 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: plasma Assay length: 3.5h Research Area: Enzyme & Kinase;Hematology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PK in the samples is then determined by comparing the OD of the samples to the standard curve. |
