ELK2175-96T, Human INHbB(Inhibin Beta B) ELISA Kit, 96T

ELK2175-96T, Human INHbB(Inhibin Beta B) ELISA Kit, 96T

ELK2176-96T, Human CALU(Calumenin) ELISA Kit, 96T

ELK2176-96T, Human CALU(Calumenin) ELISA Kit, 96T

ELK2176-48T, Human CALU(Calumenin) ELISA Kit, 48T

2.142,00 RON

Human CALU(Calumenin) ELISA Kit

SKU
ELK2176-48T

Alternative Names: Crocalbin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.263 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Developmental science;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CALU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CALU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CALU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CALU in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Crocalbin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.263 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Developmental science;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CALU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CALU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CALU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CALU in the samples is then determined by comparing the OD of the samples to the standard curve.