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ELK2155-96T, Human LDHB(Lactate Dehydrogenase B) ELISA Kit, 96T
2.963,10 RON
Human LDHB(Lactate Dehydrogenase B) ELISA Kit
SKU
ELK2155-96T
Alternative Names: LDH-B; LDH-H; Renal carcinoma antigen NY-REN-46
Species: Human
Assay Type: Sandwich
Sensitivity: 0.133 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LDHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LDHB in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: LDH-B; LDH-H; Renal carcinoma antigen NY-REN-46 Species: Human Assay Type: Sandwich Sensitivity: 0.133 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase;Cardiovascular biology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LDHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LDHB in the samples is then determined by comparing the OD of the samples to the standard curve. |
