ELK2140-48T, Human GP73(Golgi Protein 73) ELISA Kit, 48T

Alternative Names: GOLM1; GOLPH2; C9orf155; Golgi Membrane Protein 1; Golgi Phosphoprotein 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.229 ng/mL

Standard: 40 ng/mL

Detection range: 0.63-40 ng/mL

Sample type: serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Tumor immunity;Infection immunity;Reproductive science;Hepatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GP73. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GP73. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GP73, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GP73 in the samples is then determined by comparing the OD of the samples to the standard curve.