ELK2111-48T, Human C7(Complement Component 7) ELISA Kit, 48T

ELK2111-48T, Human C7(Complement Component 7) ELISA Kit, 48T

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ELK2111-96T, Human C7(Complement Component 7) ELISA Kit, 96T

2.963,10 RON

Human C7(Complement Component 7) ELISA Kit

SKU
ELK2111-96T

Alternative Names: C7 ; CO7; complement component 7 ; Complement component C7

Species: Human

Assay Type: Sandwich

Sensitivity: 0.4 ng/mL

Standard: 64 ng/mL

Detection range: 1-64 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Infection immunity;Immune molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C7 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: C7 ; CO7; complement component 7 ; Complement component C7

Species: Human

Assay Type: Sandwich

Sensitivity: 0.4 ng/mL

Standard: 64 ng/mL

Detection range: 1-64 ng/mL

Sample type: Serum, plasma and other biological fluids.

Assay length: 3.5h

Research Area: Infection immunity;Immune molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C7 in the samples is then determined by comparing the OD of the samples to the standard curve.