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ELK2094-96T, Human KLK11(Kallikrein 11) ELISA Kit, 96T
2.963,10 RON
Human KLK11(Kallikrein 11) ELISA Kit
SKU
ELK2094-96T
Alternative Names: TLSP; hK11; Hippostatin; Kallikrein-Related Peptidase 11; Serine protease 20; Trypsin-like protease
Species: Human
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLK11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLK11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK11 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: TLSP; hK11; Hippostatin; Kallikrein-Related Peptidase 11; Serine protease 20; Trypsin-like protease Species: Human Assay Type: Sandwich Sensitivity: 0.053 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids. Assay length: 3.5h Research Area: Enzyme & Kinase;Tumor immunity; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLK11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLK11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK11 in the samples is then determined by comparing the OD of the samples to the standard curve. |
