ELK2048-96T, Human PROC(Protein C) ELISA Kit, 96T

Alternative Names: PRO-C; VKDPC; PC; Vitamin K Dependent Protein C; Inactivator Of Coagulation Factors Va And VIIIa; Autoprothrombin IIA; Blood coagulation factor XIV; Activation peptide

Species: Human

Assay Type: Sandwich

Sensitivity: 24.8 pg/mL

Standard: 4000 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Plasma

Assay length: 3.5h

Research Area: Enzyme & Kinase;Hematology;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PROC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PROC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PROC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PROC in the samples is then determined by comparing the OD of the samples to the standard curve.