ELK2004-96T, Mouse ITLN1(Intelectin 1) ELISA Kit, 96T

Alternative Names: HL1; INTL; ITLN; LFR; HIntL; Omentin; Intelectin 1,Galactofuranose Binding; Intestinal Lactoferrin Receptor; Endothelial lectin HL-1

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.9 pg/mL

Standard: 160 pg/mL

Detection range: 2.5-160 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Cardiovascular biology;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITLN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITLN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITLN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITLN1 in the samples is then determined by comparing the OD of the samples to the standard curve.