ELK1968-96T, Human EPCAM(Epithelial Cell Adhesion Molecule) ELISA Kit, 96T

ELK1968-96T, Human EPCAM(Epithelial Cell Adhesion Molecule) ELISA Kit, 96T

ELK1969-96T, Mouse ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit, 96T

ELK1969-96T, Mouse ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit, 96T

ELK1969-48T, Mouse ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit, 48T

2.142,00 RON

Mouse ACACa(Acetyl Coenzyme A Carboxylase Alpha) ELISA Kit

SKU
ELK1969-48T

Alternative Names: ACAC; ACC1; ACCA; ACAC; ACC2; ACCB; ACACB; ACACA; Acetyl CoA Carboxylase; Biotin carboxylase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACACa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACACa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACACa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACACa in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: ACAC; ACC1; ACCA; ACAC; ACC2; ACCB; ACACB; ACACA; Acetyl CoA Carboxylase; Biotin carboxylase

Species: Mouse

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection range: 0.32-20 ng/mL

Sample type: Tissue homogenates, cell lysates and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACACa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACACa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACACa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACACa in the samples is then determined by comparing the OD of the samples to the standard curve.