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ELK1895-96T, Human ASM(Acid Sphingomyelinase) ELISA Kit, 96T
2.963,10 RON
Human ASM(Acid Sphingomyelinase) ELISA Kit
SKU
ELK1895-96T
Alternative Names: SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase
Species: Human
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASM in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase Species: Human Assay Type: Sandwich Sensitivity: 0.057 ng/mL Standard: 10 ng/mL Detection range: 0.16-10 ng/mL Sample type: Serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASM in the samples is then determined by comparing the OD of the samples to the standard curve. |
