ELK1836-48T, Human MCP(Membrane Cofactor Protein) ELISA Kit, 48T

Alternative Names: CD46; MIC10; TLX; TRA2; Trophoblast leukocyte common antigen; CD46 Molecule,Complement Regulatory Protein; CD46 Molecule,Complement Regulatory protein

Species: Human

Assay Type: Sandwich

Sensitivity: 4.8 pg/mL

Standard: 800 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, urine, saliva and other biological fluids

Assay length: 3.5h

Research Area: CD & Adhesion molecule;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCP in the samples is then determined by comparing the OD of the samples to the standard curve.