ELK1824-96T, Human KLF4(Kruppel Like Factor 4, Gut) ELISA Kit, 96T

Alternative Names: EZF; GKLF; Gut-Enriched Krüppel-Like Factor; Epithelial zinc finger protein EZF

Species: Human

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection range: 0.16-10 ng/mL

Sample type: Tissue homogenates and other biological fluids.

Assay length: 3.5h

Research Area: Signal transduction;Tumor immunity;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLF4 in the samples is then determined by comparing the OD of the samples to the standard curve.