Human CD14(Cluster Of Differentiation 14) ELISA Kit
Alternative Names: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
Species: Human
Assay Type: Sandwich
Sensitivity: 1.52 ng/mL
Standard: 60 ng/mL
Detection range: 0.94-60 ng/mL
Sample type: serum, plasma, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD14 in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 1.800,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein Species: Human Assay Type: Sandwich Sensitivity: 1.52 ng/mL Standard: 60 ng/mL Detection range: 0.94-60 ng/mL Sample type: serum, plasma, cell lysates and other biological fluids Assay length: 3.5h Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD14 in the samples is then determined by comparing the OD of the samples to the standard curve. |