ELK1776-96T, Human LCN1(Lipocalin 1) ELISA Kit, 96T

Alternative Names: TP; PMFA; VEGP; Tlc; Tear Prealbumin; Tear lipocalin; Protein Migrating Faster Than Albumin; Von Ebner Gland Protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.62 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: serum, plasma, saliva, tears and other biological fluids and other biological fluids

Assay length: 3.5h

Research Area: Metabolic pathway;Infection immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LCN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LCN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LCN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LCN1 in the samples is then determined by comparing the OD of the samples to the standard curve.