ELK1732-48T, Human a1ACT(Alpha-1-Antichymotrypsin) ELISA Kit, 48T

Alternative Names: A1-ACT; SERPINA3; AACT; ACT; GIG24; GIG25; Serpin Peptidase Inhibitor Clade A Member 3(Alpha-1 Antiproteinase; Antitrypsin); Cell growth-inhibiting gene 24/25 protein

Species: Human

Assay Type: Sandwich

Sensitivity: 1.47 ng/mL

Standard: 200 ng/mL

Detection range: 3.13-200 ng/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Neuro science;Pulmonology;Hepatology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a1ACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a1ACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a1ACT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a1ACT in the samples is then determined by comparing the OD of the samples to the standard curve.