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ELK1722-96T, Mouse CFH(Complement Factor H) ELISA Kit, 96T
2.963,10 RON
Mouse CFH(Complement Factor H) ELISA Kit
SKU
ELK1722-96T
Alternative Names: CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
Species: Mouse
Assay Type: Sandwich
Sensitivity: 3.7 ng/mL
Standard: 500 ng/mL
Detection range: 7.82-500 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CFH in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein Species: Mouse Assay Type: Sandwich Sensitivity: 3.7 ng/mL Standard: 500 ng/mL Detection range: 7.82-500 ng/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Infection immunity;Immune molecule; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CFH in the samples is then determined by comparing the OD of the samples to the standard curve. |
