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ELK1712-48T, Rat COL4(Collagen Type IV) ELISA Kit, 48T
2.142,00 RON
Rat COL4(Collagen Type IV) ELISA Kit
SKU
ELK1712-48T
Alternative Names: Type-IV Collagen
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.269 ng/mL
Standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Type-IV Collagen Species: Rat Assay Type: Sandwich Sensitivity: 0.269 ng/mL Standard: 40 ng/mL Detection range: 0.63-40 ng/mL Sample type: serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Tumor immunity;Hepatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
