ELK1686-96T, Rat TSP-1(Thrombospondin-1) ELISA Kit, 96T

Alternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 3.6 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: Plasma

Assay length: 3.5h

Research Area: Infection immunity;Hematology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.