ELK1650-96T, Mouse ANXA5(Annexin A5) ELISA Kit, 96T

ELK1650-96T, Mouse ANXA5(Annexin A5) ELISA Kit, 96T

ELK1651-96T, Rat REN(Renin) ELISA Kit, 96T

ELK1651-96T, Rat REN(Renin) ELISA Kit, 96T

ELK1651-48T, Rat REN(Renin) ELISA Kit, 48T

2.142,00 RON

Rat REN(Renin) ELISA Kit

SKU
ELK1651-48T

Alternative Names: angiotensinogenase

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.7 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat REN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat REN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat REN in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: angiotensinogenase

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.7 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat REN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat REN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat REN in the samples is then determined by comparing the OD of the samples to the standard curve.