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ELK1651-48T, Rat REN(Renin) ELISA Kit, 48T
2.142,00 RON
Rat REN(Renin) ELISA Kit
SKU
ELK1651-48T
Alternative Names: angiotensinogenase
Species: Rat
Assay Type: Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat REN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat REN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat REN in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 1.800,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: angiotensinogenase Species: Rat Assay Type: Sandwich Sensitivity: 5.7 pg/mL Standard: 1000 pg/mL Detection range: 15.63-1000 pg/mL Sample type: serum, plasma and other biological fluids Assay length: 3.5h Research Area: Endocrinology;Cardiovascular biology;Kidney biomarker;Hormone metabolism; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat REN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat REN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat REN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat REN in the samples is then determined by comparing the OD of the samples to the standard curve. |
