ELK1642-96T, Rat CC16(Clara Cell Protein 16) ELISA Kit, 96T

Alternative Names: SCGB1A1; CC10; CCSP; UGB; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 30 pg/mL

Standard: 5000 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: serum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Infection immunity;Cardiovascular biology;Pulmonology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CC16. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CC16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CC16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CC16 in the samples is then determined by comparing the OD of the samples to the standard curve.