Human CETP(Cholesteryl Ester Transfer Protein) ELISA Kit
Alternative Names: LTP; Lipid Transfer Protein; Plasma Lipid Transfer Protein; Cholesterylester Transfer Protein
Species: Human
Assay Type: Sandwich
Sensitivity: 1.31 ng/mL
Standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CETP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CETP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CETP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CETP in the samples is then determined by comparing the OD of the samples to the standard curve.
Price | 1.800,00 RON (preturile sunt fara TVA) |
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Description |
Alternative Names: LTP; Lipid Transfer Protein; Plasma Lipid Transfer Protein; Cholesterylester Transfer Protein Species: Human Assay Type: Sandwich Sensitivity: 1.31 ng/mL Standard: 200 ng/mL Detection range: 3.13-200 ng/mL Sample type: serum, plasma, tissue homogenates and other biological fluids Assay length: 3.5h Research Area: Metabolic pathway;Hepatology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CETP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CETP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CETP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CETP in the samples is then determined by comparing the OD of the samples to the standard curve. |