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ELK1554-96T, Rat TAT(Thrombin/Antithrombin Complex) ELISA Kit, 96T
2.963,10 RON
Rat TAT(Thrombin/Antithrombin Complex) ELISA Kit
SKU
ELK1554-96T
Alternative Names: Thrombin/Antithrombin Complex
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.117 ng/mL
Standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TAT in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 2.490,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: Thrombin/Antithrombin Complex Species: Rat Assay Type: Sandwich Sensitivity: 0.117 ng/mL Standard: 20 ng/mL Detection range: 0.32-20 ng/mL Sample type: Plasma Assay length: 3.5h Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;Hematology; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TAT in the samples is then determined by comparing the OD of the samples to the standard curve. |
