ELK1509-96T, Rat TP53(Tumor Protein p53) ELISA Kit, 96T

ELK1509-96T, Rat TP53(Tumor Protein p53) ELISA Kit, 96T

ELK1510-96T, Rat SP(Substance P) ELISA Kit, 96T

ELK1510-96T, Rat SP(Substance P) ELISA Kit, 96T

ELK1510-48T, Rat SP(Substance P) ELISA Kit, 48T

2.142,00 RON

Rat SP(Substance P) ELISA Kit

SKU
ELK1510-48T

Alternative Names: Substance P

Species: Rat

Assay Type: Sandwich

Sensitivity: 4.79 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Neuro science;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SP in the samples is then determined by comparing the OD of the samples to the standard curve.

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Description

Alternative Names: Substance P

Species: Rat

Assay Type: Sandwich

Sensitivity: 4.79 pg/mL

Standard: 1000 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Neuro science;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SP in the samples is then determined by comparing the OD of the samples to the standard curve.