ELK1503-48T, Rat NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit, 48T

Alternative Names: NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.62 ng/mL

Standard: 100 ng/mL

Detection range: 1.57-100 ng/mL

Sample type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.