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ELK1497-96T, Pig HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit, 96T
3.736,60 RON
Pig HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit
SKU
ELK1497-96T
Alternative Names: HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
Species: Pig
Assay Type: Sandwich
Sensitivity: 0.61 ng/mL
Standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Tumor immunity;Infection immunity;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HSPA1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HSPA1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HSPA1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HSPA1A in the samples is then determined by comparing the OD of the samples to the standard curve.
| Price | 3.140,00 RON (preturile sunt fara TVA) |
|---|---|
| Description |
Alternative Names: HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70 Species: Pig Assay Type: Sandwich Sensitivity: 0.61 ng/mL Standard: 100 ng/mL Detection range: 1.57-100 ng/mL Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids Assay length: 3.5h Research Area: Signal transduction;Tumor immunity;Infection immunity;Developmental science; Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HSPA1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HSPA1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HSPA1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HSPA1A in the samples is then determined by comparing the OD of the samples to the standard curve. |
