ELK1466-96T, Human AQP1(Aquaporin 1, Colton Blood Group) ELISA Kit, 96T

Alternative Names: AQP-CHIP; CHIP28; CO; Channel-Forming Integral Protein,28kDa; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule

Species: Human

Assay Type: Sandwich

Sensitivity: 0.09 ng/mL

Standard: 16 ng/mL

Detection range: 0.25-16 ng/mL

Sample type: Serum, plasma, tissue homogenates and other biological fluids

Assay length: 3.5h

Research Area: Hormone metabolism;Bone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AQP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AQP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AQP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AQP1 in the samples is then determined by comparing the OD of the samples to the standard curve.