ELK1453-48T, Human VIP(Vasoactive Intestinal Peptide) ELISA Kit, 48T

Alternative Names: PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27

Species: Human

Assay Type: Sandwich

Sensitivity: 2.63 pg/mL

Standard: 500 pg/mL

Detection range: 7.82-500 pg/mL

Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length: 3.5h

Research Area: Endocrinology;Gastroenterology;Hormone metabolism;

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VIP in the samples is then determined by comparing the OD of the samples to the standard curve.